Synthesis Of Three Analogues Of Trypsin-Modulating Oostatic Factor TMOF And Screening Of Their Insecticidal Properties Towards Cabbage Cluster Caterpillar

Three analogues of TMOF PP H-Pro-Pro-OH PPPPPP H-Pro-Pro-Pro-Pro-Pro-Pro-OH and APPPPPP H-Pro-Pro-Pro-Pro-Pro-Pro-OH were successfully synthesised and screened for their insecticidal properties against cabbage cluster caterpillar Crocidolomia pavonana. All compounds were prepared through Fmoc-based solid-phase peptide synthesis SPPS method. The synthesis employed chlorotrityl resin as solid support. A combination of NN-diisopropylcarbodiimide DIC and ethyl 2-cyano-2-hydroxyiminoacetate oxyma was chosen as coupling reagent in the synthesis. The peptide was cleaved by using TFA cocktail. All peptide crudes were purified by using reversed-phase flash chromatography with octadecyl silane ODS as stationary phase. All purified peptides were characterized by using TOF-ESMS and their purity was analysed using thin layer chromatography and analytical RP-HPLC. The synthesised TMOF analogues together with TMOF were biologically tested towards the C. pavonana at 1000 ppm. The results indicated that TMOF and the analogues were less effective for the cabbage cluster caterpillar compared to previously reported activity of TMOF towards Aedes aegypty.