Sterilization Method For In Vitro Propagation Explant Embryo Of Durio Kutejensis Hassk. 26 Becc From Kalimantan

Durio kutejensis the local durian forest result Kalimantan that has not been widely known outside of the island and not much cultivated. One of the obstacles of plant propagation is seedling superior quality are not available. Propagation through the technique of in vitro Tissue culture in plants Durio kutejensis Hassk 26 Becc not been widely tested because the woody plants have grown a great degree of difficulty. Commonly cultivated plants through in vivo techniques. Sterilization is an important part of the culture in vitro. Sterilization of models have been tried to overcome the initial barriers of culture. The development of in vitro embryo culture D. kutejensis through embryo explants using Murashige and Skoog MS with the addition of growth regulators GA3 gibberellic acid and BAP Benzilaminopurine produces elongation of hypocotyl added shoot growth and root elongation. Callus formed from embryonic explants green and yellowish white with compact texture on the addition of growth regulators BAP and 2.4 D 1grl. Callus formation has not been followed by the development of embryogenesis and not yet differentiation into shoots and roots.