Study On The Effects Of Heavy Metals Pollution On The Activity Of 7-Ethoxyresorufin-O-Diethylase EROD In Two Freshwater Fish Species Of Indonesia
Journal: International Journal of Scientific & Technology Research (Vol.6, No. 6)Publication Date: 2017-06-15
Authors : Nur Kusuma Dewi;
Page : 173-178
Keywords : EROD activity; heavy metals; biomarker; river environment;
Abstract
The study aimed to determine the effect of heavy metal pollution on 7-ethoxyresorufin-O-diethylase EROD enzyme activity in carp Cyprinus carpio and tilapia Oreochromis niloticus. The samples were taken by purposive random sampling technique. The treatment site was the downstream part of the Kaligarang River Semarang Indonesia which contaminated byheavy metals concentration of Cd 0.007 ppm Pb 0.010 ppm and Hg 0.0006 ppm. A clean reference site in Nyatnyono Village Ungaran was used as the control site with the heavy metal content of 0 ppm. The EROD activities in carp and tilapia were significantly decreased after Cd Pb and Hg exposure. The results showed that the average value of liver EROD activity in carp fish control group amounted to 1.77 0.23molminmg protein and the treatment group was 0.49 0.24molminmg protein. Whereas in control group of tilapia fish EROD activity was equal to 2.08 0.47molminmg protein and the treatment group was 0.49 0.40molminmg protein. The results demonstrated that metallothionein was a specific heavy metals biomarker whereas EROD activity ishighly sensitive to extremely low concentrations of theselected pollutants.In conclusion the use of multiple biomarkers is recommended to monitor the heavy metal pollutants in the river environment.
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