Antioxidant Chemo-Protective Role Of Buffalo Colostrum And Milk Whey Derived Peptide Against 2 4-Dinitrophenol Induced-Oxidative Damage On Human Plasma In Vitro.

This study investigates the role of buffalo colostrum and milk whey derived peptides in protection against oxidative damage induced by 2 4-Dinitrophenol 2 4-DNP in human blood serum samples in vitro. A biomarker enzymes for oxidative stress like Alkaline phosphatase ALP and acid phosphatase ACP oxidative damage markers indicating extent of host antioxidant reserve indicators like reduced glutathione GSH were measured by spectrophotometric techniques in four different groups namely 1 Human blood plasma only control 2 Human blood plasma 24-DNP 3 Human blood plasma colostrum whey derived peptides 24-DNP 4 Human blood plasma colostrum whey derived peptides 5 Human blood plasma milk whey derived peptides 24-DNP and 6 Human blood plasma milk whey derived peptides. Following exposure to 2 4-DNP levels of antioxidants like GSH was significantly decreased in comparison to control e.g. GSH 0.568 0.015 vs0.871 0.022mol0.1 mg proteins. In addition the concentration of biomarker enzymes for 24-DNP induced membrane damage and oxidative stress like ALP and ACP were increased in serum by oxidant compared to control e.g. ALP 5.497 0.185 vs. 2.782 0.000molmg protein ACP 1.689 0.047 vs. 0.629 0.047molmg protein. Pretreatment with buffalo whey derived peptides significantly protects 24-DNP induced RBC membrane lyses and release of ALP and ACP into serum environment. e.g. ALP 3.444 0.094 vs. 5.497 0.185 molmg protein ACP 0.629 0.047 vs. 1.689 0.047molmg protein. Pretreatment with whey derived peptides give protection to oxidative damage and shifts the trend towards amelioration and replenishment of the antioxidant status.