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Identification of Erythrobactor Sp. Strains By 16S Ribosomal RNA Sequence Analysis

Journal: International Journal of Scientific & Technology Research (Vol.1, No. 6)

Publication Date:

Authors : ; ; ;

Page : 117-122

Keywords : KeywordsEzTaxon; Erythrobactor; Polymerase chain reaction; Taxonomy and 16S rRNA;

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Abstract---16S ribosomal RNAs rRNA of 4 Erythrobacter strains have been almost completely sequenced to establish their phylogenetic relationships. They have shown growth only at 25oC. 16S rRNA sequences of these strains have shown good similarity with Erythrobacter nanhaisediminis T30 T and E. citreus RF35F 1T in EzTaxon identity analysis results. 16S rRNA fragment of A3e5 A3e7 and D2g2 amplified with 27F and 907R primers were most closely related with E. citreus RF35F1T and they were represented on same branch along with E. citreus RF35F1T. A3e7 Np292 and D2g2s 16S rRNA fragment amplified with 518F and 1513R primers were closely related with E. nanhaisediminis T30T strain. Sequences with more than 97 similarity with existed database sequences were considered. Using these sequences and most closely related sequences from data base were used for drawing phylogenetic tree.

Last modified: 2013-04-13 21:39:04