Production Purification Characterization and Comparison of Polygalacturonase from various strains of Aspergillus
Journal: International Journal of Scientific & Technology Research (Vol.1, No. 9)Publication Date: 2012-10-25
Authors : Niketan Deshmukh; Reshma Talkal; Kaushik Jha; Praveen Gaurav Singh; Deep Chandra Prajapati;
Page : 85-91
Keywords : Keywords- Polygalacturonase PG; Submerged fermentation; Aspergillus niger; Aspergillus flavus; Aspergillus oryzae; Ammonium sulfate;
Abstract
Abstract- Polygalacturonase is an important enzyme used in food and chemical industries that processed plant material like Juice extraction Clarification of wine Textile etc. Fungi from genus Aspergillus are one of the most important sources of this enzyme. We compare three strains of Aspergillus Aspergillus niger Aspergillus flavus and Aspergillus oryzae for production Polygalacturonase by submerged fermentation. We also characterized all the Polygalacturonase isolated from different strains at different parameters such as pH Temperature Incubation time Concentration Substrate in medium Concentration of enzyme etc and on the basis of these parameters it is observed that Aspergillus niger found to better strain then Aspergillus flavus and Aspergillus oryzae.
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