Ultrastructure Germination And Viability In Pollens Of Achillea Wilhelmsii C.Koch Exposed To Electromagnetic Fields
Journal: International Journal of Scientific & Technology Research (Vol.2, No. 2)Publication Date: 2013-02-25
Authors : Ahmad Majd; Leila Amjad; Azadeh Ghadirianmarnani;
Page : 103-107
Keywords : Index Terms - electromagnetic field; germination; pollen; viability.;
Abstract
Abstract - Electromagnetic field is an inevitable environmental factor for living beings which many investigations have been conducted to consider its effect. In this research the effects of electromagnetic field on pollen ultrastructure pollen germination and viability of Achillea wilhelmsii were studied. Achillea wilhelmsii C. Koch was collected from around area of Isfahan city Iran. In case of pollen treatments the pollens were exposed to electromagnetic field by a magnitude of 24 mT at 10 min and 24 mT at 20 min in the electromagnetic field generator apparatus. Untreated pollens were used as control under similar condition. Pollens were studied by Scanning electron microscopy. The effect of electromagnetic fields on pollen germination was evaluated. Germination was quatified as the percentage of germinated pollen grains per 100 evaluated. Pollen grains for study of viability were stained with Evans blue. The results were analyzed using ANOVA statistical to analyses of the treatments. Treatment under pollens with electromagnetic fields 2mT-1020min have few changes but treatment under pollens with electromagnetic fields 4mT-20min become abnormal shrinkage and fragile. the percentage of pollen germination and viability decreased that was significantlyp 0.05. electromagnetic field have effects on the structure of some organs and developmental characteristic of them in Achillea wilhelmsii. Therefore a direct influence of environmental stresses is discussed affecting Ca2 levels via the ion cyclotron resonance mechanism.
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