Production And Partial Characterization Of a Thermostable Alkaline And Organic Solvent Tolerant Lipase From Trichoderma atroviride 676

Abstract The several industrial applications of lipases have stimulated interest in isolation of new enzymes from novel sources. In this study the lipase production from Trichoderma atroviride 676 was optimized by statistical design methods. The response surface methodology based on 33-1 fractional factorial showed that the yeast extract and MgSO4 played a significant role on lipase production yielded an enzymatic activity of 101.75 Uml. Using Box-Benhken design the optimization of the temperature levels pH and agitation rate resulted in the maximum enzyme production of 175.20 Uml obtained at 28 C pH 6.0 and 105 rpm. The enzyme was optimally active at pH 8.0 and 35C and was stable at pH 3.0-8.0 and temperature of 25C-75C. In addition the lipase was highly stable on non-polar hydrophobic solvents as kerosene n-dodecane and hexane. The new lipase from T. atroviride 676 could be considered a potential candidate for industrial and biotechnological applications.