In Vitro Morphogenesis Of Colysis LatilobaChing. Ching

Abstract - Spores of the fern Colysis latiloba reared in Knops medium with various concentration of sucrose ranging from 0 to 6 germinated after two weeks. Only 15 spores germinated in the medium without sucrose. Maximum spore germination 86 was observed in the medium with 1 sucrose. Pre-germination stages were almost similar in all the cases. This species exhibited bipolar germination. The gametophyte was thalloid dorsoventrally flattened and spatulate. Sex organs were not observed up to 23 weeks which indicates development of apogamous sporophyte.