COMPARATIVE STUDY ON METHANOLIC AND CHLOROFORM EXTRACTS OF TRACHYSPERMUM AMMI SEEDS FOR ANTIARTHRITIC AND ANTIGOUT ACTIVITY

Objective: The objective of the present study is to report the in vitro anti-arthritic and anti-gout activity of methanolic and chloroform extract of Trachyspermum ammi seeds. Materials and Methods: Fresh Trachyspermum ammi seeds were collected and extracted by using methanol and chloroform solvent by successive soxhelation for the constituents. Preliminary phytochemical screening has been done. Methanolic extract was tested for its polyphenolic content by Folin-ciocalteu's reagent. In vitro anti-arthritic activity was performed by inhibition of protein denaturation method. In vitro anti-gout activity was performed by the metal chelating complex assay. Results: The study showed the presence of cardiac glycosides, carbohydrates, phytosterols, saponins, phenolic and tannins in the methanolic extract. Alkaloids, saponins, phenolics, tannins, carbohydrates in chloroform extract. The methanolic extract showed significant anti-arthritic activity than chloroform extract, this might be due to methanolic extract was rich in polyphenols. The anti-gout activity of Trachyspermum ammi seeds methanolic extract was also observed to be highly significant in its activity. Conclusion: Both the methanolic and chloroform extracts of Trachyspermum ammi seeds were found to have significant anti-arthritic and anti-gout activity when compared to standard drug diclofenac in a dose-dependent manner.