SIMULTANEOUS ESTIMATION OF AMLODIPINE AND ROSUVASTATIN IN COMBINED BULK FORMS BY RP-HPLC USING ULTRAVIOLET DETECTION

The objective of the study was to develop simple RP-HPLC method for the simultaneous determination of amlodipine and rosuvastatin. In this method, kromasil C18 (100 mm, 4.6 mm, 5 ?m) column was used. The mobile phase and flow rate used were {(acetonitrile 40, 55, 70, 40, 40): (phosphate buffer 60, 45, 30, 60, 60)}, (Time 0.5, 2.0, 3.0, 3.0, 2.0). UV detection was monitored at 239 nm. Calibration graphs were established for amlodipine and rosuvastatin respectively. The average retention time for amlodipine and rosuvastatin was found to be 2.40±0.16 min and 4.28±0.04 min, respectively. The intraday and Interday precision expressed as percent relative standard deviation was below 2%. The validated HPLC method was found to be rapid, precise and accurate and can be readily utilized for analysis of amlodipine and rosuvastatin in bulk forms.