Studied of CD14 and CD15 Gene Expressions for Myeloid Derived Suppressor Cell Profile at the RNA Level as a Predictor for Progressivity in Nasopharyngeal Carcinoma

Background: Nasopharyngeal carcinoma (NPC) is a disease in which malignant cells formed in the tissue of the nasopharynx, which is a frequent cancer in Indonesia. Estimated of NPC in Indonesia at 6.2/100.000 or about 12.000 new cases per year. Developments of tumors are influenced by many factors, generally an interaction between genetic, environmental and immune system. There is a role of myeloid derived suppressor cells in the process of immune suppression. Objective: The aim of this study is to clarify the profile of MDSC encoded by CD14 and CD15 genes expression to find the predictor for progressivity of nasopharyngeal carcinoma. Peripheral blood specimen and biopsy from primary tumor were collected from 16 nasopharyngeal carcinoma patients. The samples collected underwent qRT-PCR. Data were analyzed by 2-ΔΔCt methods and statistical analysis. Results: There were up regulation of CD14 and CD15 m-RNA level in blood at advanced stage (52.42 and 38.03) using 2-ΔΔCt methods. The expression of CD14 and CD15 genes was strongly correlated with clinical stage. Conclusion: MDSC can be used as a predictor of progression in NPC.