Russell´s viper (Daboia Russelli Russelli) Venom Toxicity Neutralizing Efficacy of Curcumin- Gold Nanoparticle (C-GNP) in Experimental Animal Model

Herbs/herbal compounds possess anti-snake venom activity. Anti-snake venom activity of Curcuma longa in animal model has been reported earlier. In the present study, Curcumin (C), an active ompound present in Curcuma longa was Conjugated with Gold Nanoparticle (C-GNP) and its neutralizing efficacy against viper (Daboia russelli russelli) venom induced toxicity was evaluated in animal models. C-GNP was synthesized by adsorption method using chemically synthesized gold nanoparticle. Physicochemical characterization of C-GNP was done by DLS (size + zeta potential), UV-visible spectra, XRD and FESEM. Snake venom neutralizing activity of curcumin/C-GNP was done using in vivo and in vitro models. Animals were divided into gr. 1: Sham control, gr. 2: RVV control, gr. 3: Curcumin treated and gr. 4: C-GNP treated. Animal ethical clearance was availed before experiments (IAEC/IV/Proposal/AG- 001/2015; Dt: 10.04.2015). One way ANOVA was done (n=4) and Pin vitro) was observed after C-GNP treatment. The present study confirmed the formation of C-GNP and its efficacy against RVV induced toxicity. It may act as supportive treatment of snake bite victims. Further detailed studies on the antivenom activity and molecular mechanism of C-GNP are warranted.