Polypeptide Profiles of Diminazene Aceturate Resistant Trypanosoma evansi Organisms Isolated from a Buffalo

Trypanosomosis was recorded in a buffalo during the month of September 2014 in Proddatur of Andhra Pradesh, India. Disease was confirmed by the presence of Trypanosoma evansi organisms in peripheral blood smears. Buffalo was treated with intra muscular administration of diminazene aceturate at the rate of 3.5 mg perkg body weight along with supportive therapy. After two days of therapy, re-examination of blood smear revealed the presence of live Trypanosoma evansi organisms. To determine the profiles of diminazene resistant Trypanosoma evansi organism's whole blood was collected and processed. Whole cell lysate antigen was prepared from the host cell free Trypanosoma evansi parasites. A total of 8 polypeptide bands with relative molecular weight of 34, 48, 53, 55, 64, 74, 80 and 98 kDa were identified. Buffalo was successfully treated with sub cutaneous administration of antrycide prosalt, at the dose rate of .5 mg per kg body weight along with supportive therapy.