IN VITRO CYTOTOXICITY EVALUATION OF POLYMERIZED RESIN CEMENTS ON THE VIABILITY OF L-929 FIBROBLAST CELLS

Objective: This study was designed to evaluate the cytotoxicity of two resin-based cements; Bistite II DC, and Multi Bond II, after polymerization on cultured L-929 fibroblast cells. Methods: Disc-shaped samples were prepared in polyethylene molds with cylindrical cavities (5 mm diameter, 2 mm high) of BistiteII DC and Multi Bond II. After setting of resin cement disc, they were aged for 1, 3, and 5 days in Dulbecco's Modified Eagle's Medium (DMEM). Cell viability of L-929 fibroblast cells was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the difference between the groups was tested by analysis of variance and Tukey tests (a = 0.05). Result: After 1 day of storage, the Bistite II DC, and Multi Bond II were essentially non-cytotoxic. On days 5 of the experiment, the cell viability of two resin cements did not differ significantly (P > 0.05), but cell viability was slightly reduced on day 5. There was no significant difference in the cytotoxicity between Bistite II DC, and Multi Bond II. Conclusion: After polymerization, two resin-based cements (Bistite II, and Multi Bond) induced slight cytotoxicity. The sensitivity of cytotoxicity to L-929 cells in depended on the type of resin-based cements.