Bacteriological Quality Assessment of Some Locally Manufactured Dairy Desserts Sold in Beni-Suef City, Egypt and Molecular Detection of Staphylococcus aureus Enterotoxin Genes

Seventy-five randomly collected samples of ice-cream, rice with milk and mehallabeia (25, each) from different localities in Beni-Suef city, Egypt, were bacteriologically examined. The mean values of aerobic plate, Coliforms, Fecal coliforms, E. coli, Enterococci, Bacillus cereus and Staphylococcus aureus counts/g in the examined ice-cream samples were 7.02×105±4.93×105, 1.45×105±3.21×104, 9.58×102±4.6×102, 1.35×102±66, 8.12×104±4.61×104, 1.17×104±4.59×103 and 1.51×104±9.13×103, while, in rice with milk samples they were 5.52×104±2.78×104, 3.52× 103±1.94 ×103, 1.66×102±1.56×102,4.8±3.1, 1.28×104±6.25×103, 2.38×105±1.4×105 and 6.32×102±2.96×102, respectively. In mehallabeia samples, the counts were 6.07×104 ±3.6×104, 6.59×103±2.6×103, 1.6×102±89, 9.48±8, 2.6×104±1.64×104, 3.83×105±3.27×105 and 4.89×102±3.28×102, respectively. Amplification of enterotoxigenic associated genes using multiplex PCR, showed that sea and see genes were detected in all the examined coagulase positive S. aureus isolates. In conclusion, strict hygienic measures should be followed during production and handling of milk products with health educational programs for producers and handlers.