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Development of Liquid Chromatographic Method for Estimation of Ondansetron and Ranitidine in Combined Dosage Form

Journal: Austin Chromatography (Vol.2, No. 2)

Publication Date:

Authors : ; ; ; ;

Page : 1-6

Keywords : Ondansetron; Ranitidine; RP-HPLC; Validation; Degradation studies;

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Abstract

Isocratic, reversed phase liquid chromatographic method was developed for the quantitative determination of Ondansetron and Ranitidine in combined dosage form. A Phenomenex Luna C18, 5 μm column with mobile phase containing methanol: water (60:40) was used. The flow rate was 1.0 ml/min and analytes were monitored at 299 nm. The retention times of ondansetron and ranitidine were 9.9 min and 5.5 min, respectively. The linearity for ondansetron and ranitidine were in the range of 0.05-10 μg/ ml and 0.05 - 60 μg/ ml, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity and robustness. Stock solution was subjected to acid and alkali hydrolysis, chemical oxidation, dry heat degradation and photo degradation stability assessment. The method was successfully applied to the estimation of ondansetron and ranitidine in combined dosage form.

Last modified: 2017-02-15 19:10:38