The Association of Set3 Complexes with Nucleosomes and whole Genome in Saccharomyces Cerevisiae
Journal: Austin Journal of Computational Biology and Bioinformatics (Vol.1, No. 1)Publication Date: 2014-05-10
Authors : Tung SY; Tsai HC; Shen HH; Tsai SP; Lee SP; Hsiao SP; Chen FJ; Liou GG;
Page : 1-9
Keywords : ChIP; Genome-wide; Protein-protein interaction; Nucleosome; Set3; SET3C;
Abstract
The Set3 Complex (SET3C) is involved in several biological processes such as transcription. SET3C is capable of function not only in the repression of gene expression but also in the activation. To characterize the molecular property of SET3C in more detail, here, we used a typical TAP tag method and succeed to directly purify a native Set3-TAP complex from yeast and so did Snt1-TAP complex. Both the components of Set3-TAP complex and of Snt1-TAP complex were same as the reported model of SET3C, including the proteins Snt1, Hos4, Set3, Sif2, Hos2, Hst1 and Cpr1. Furthermore, we also demonstrated that the SET3C was able to associate with nucleosome by several biophysical methods. And through the Chromatin Immunoprecipitation (ChIP) on chip assay, we also analyzed 4 data sets of the genome-wide localization of SET3C to identify 590 major association gene regions of SET3C. In summary the positive and negative role on the modulation of gene expression could consist with the association of SET3C with native nucleosomes and also agree with the exploration of dynamic global genome association of SET3C.
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