Validation of Method for Identifying Aromatic Amines from Dyed Leather

The use of chemicals to obtain semi-processed leather must be consistent with the environmental laws and regulations, which requires evaluation of toxicity in leather goods intended for consumption. The paper proposes to implement and validate an instrumental analytical method to determine azo dyes in leather. The presence of azo dyes was determined on a total of three restricted aromatic amines (4-aminobiphenyl, benzidine, 5-nitro-o-toluidine), which can be found as a result of the split of dyes used in leather dyeing. Validation parameters of gas chromatographic method were: accuracy (trueness and reliability), precision, sensitivity, limit of detection, limit of quantification, working range, linearity.