Determination of Hydroxyproline in Collagen Biomaterials for Medical Use and Validation of Method
Journal: Revista de Pielarie Incaltaminte / Leather and Footwear Journal (Vol.16, No. 2)Publication Date: 2016-06-30
Authors : Gabriela MACOVESCU; Ciprian CHELARU; Mădălina Georgiana ALBU KAYA; Luminiţa ALBU;
Page : 147-162
Keywords : biomaterials; hydroxyproline; validation of method; collagen;
Abstract
The paper presents a method for determining hydroxyproline in collagen biomaterials for medical use produced in The Collagen Department of INCDTP ? Division ICPI and its validation. The method has two stages: the first one consists in hydrolysis of proteins in the sample of protein product to the form of amino acids, among which hydroxyproline. The second stage refers to selective highlighting of hydroxyproline using a specific colour reaction and quantitative assessment against a reference standard with known concentration. The method was validated to establish performance parameter and to check compliance with the set goal.
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