Checkpoint kinase (Chk1) and reactive oxygen species induced oncotic cell death during the cell cycle in LLC-PK1 cells
Journal: International Journal of Biosciences (IJB) (Vol.2, No. 6)Publication Date: 2012-06-10
Authors : Hashimul Ehsan;
Page : 24-31
Keywords : Hydroquinone; Chk1; cell death; TGHQ; FCM analysis.;
Abstract
2,3,5?tris-(Glutathion-S-yl)hydroquinone (TGHQ) is a reactive oxygen species (ROS) generating metabolite of the nephotoxicant, hydroquinone. In renal proximal tubule epithelial cells (LLC-PK1), TGHQ catalyzes the formation of single strand breaks in DNA, followed by growth arrest and oncotic/necrotic death during the cell cycle. Treatment of synchronized LLC-PK1 cells with TGHQ causes an initial decrease in the expression of the DNA damage inducible protein kinase chk1. The degradation of chk1 in highly synchronized LLC-PK1 cells might induce mitotic catastrophe, a potential mechanism of cell death in this model.
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