Effect of Activators on Crude α-amylase produced by Brevibacillus borstelensis R1 isolated from coastal area of Bay of Bengal, Visakhapatnam

ABSTRACT Alpha amylase have many applications in Ethyl alcohol production, Treatment of Sago and Rice effluent, Sewage water treatment and fodder production, Textile industry, Glucose Industry, Chocolate Syrup industry, Building product industry, Feed Industry, Unmalted cereal liquefaction industry, Manufacture of high fructose containing syrups, Manufacture of maltotetraose syrup, Hydrolysis of starch to maltodextrins etc. All the activators studied showed more activity when compared with the control. The average enzyme activity of the crude enzyme found in CaCO3(427±13-2223±9 U/ml), CaCl2 (663±9- 1813±13 U/ml), CaSO4 (663±9-1430±6 U/ml), MgCl2 (570±15-733±18 U/ml), MgS04 (577±15-707±7 U/ml) and NaCl (440±20- 670±6 U/ml).The highest amylase activity was observed in CaCO3 (2223±9 U/ml) at 0.5M and lowest (427±13 U/ml) in CaCO3 at 0.2M.