PRODUKSI BIOETANOL DARI ALKALI-PRETREATMENT JERAMI PADI DENGAN PROSES SIMULTANEOUS SACHARIFICATION AND FERMENTATION (SSF)

Rice straw is an agricultural waste which contains 39% cellulose and 27.5% hemicelluloses. Rice straw can be converted into bio ethanol by Simultaneous Saccharification Fermentation (SSF) process. The aims of this research are to investigate the influence of rice straw pretreatment and operation condition (number of cellulose enzyme and Saccharomyces cereviseae) for bioethanol production. The bioethanol conversion was devided by 2 steps, there were delignification and SSF. Delignification process was done by soak rice straw in NaOH 2% heated at temperature 85 oC for 1 hour then washed with water. The pretreatment rice straw was used as substrate in SSF. SSF was conducted in the presence of cellulase enzyme (20, 30, and 40 FPU) and Sacharomyces Cerevisiae (2,4 and 6 ose) for 3 days. The bioethanol concentration produced for 20 FPU, 30 FPU, and 40 FPU in 2 ose S.careviseae are 0,45%, 0,44%, and 0,43% respectively. The addition number of Saccharomyces cereviseae was gave high concentration of bioethanol. The result shown that bioethanol concentration of 2 ose, 4 ose and 6 ose are 0,45%, 0,46% and 1,07%, respectively. In the same concentration of enzyme (20 FPU) which pretreatment and non pretretament substrate was increased of bioethanol concentration up to 82,2%. The pretretment process was broken the structure of lignin and made enzyme easy to attached cellulose and converted to glucose.