In vivo EFFECTS OF RARE-EARTH BASED NANOPARTICLES ON OXIDATIVE BALANCE IN RATS

The purpose of the research was to find the influence of rare-earth based nanoparticles (CeO2, GdVO2: Eu3+) on the oxidative balance in rats. We analyzed biochemical markers of oxidative stress (lipid peroxidation level, nitric oxide metabolites, sulfhydryl groups content) and enzyme activities (superoxide dismutase, catalase) in tissues of rats. It has been found that administration of both types of the nanoparticles increased nitric oxide metabolites and products of lipid peroxidation in liver and spleen within 5 days. At injections of GdVO2: Eu3+ lipid peroxidation products, nitric oxide metabolites in serum at 5, 10 and 15 days of the experiment was also increased whereas the level of sulfhydryl groups decreased compared to the intact state and the control. In contrast, under the influence of nanoparticle CeO2 level diene conjugates were not significantly changed and the level of nitric oxide metabolites within 15 day even decreased. During this period, under the influence of both types of nanoparticles the activity of superoxide dismutase was increased, catalase activity was not changed. Oxidative stress coefficient showed the less pronounced CeO2 prooxidant effect (2.04) in comparison to GdVO2: Eu3+ (6.89). However, after-effect of both types of nanoparticles showed complete restoration of oxidative balance values.