Conserved Domain Analysis of Photorhabdus luminescens Strain TTO1 Hypothetical Proteins Encoding Enzyme Activity

Aim: Photorhabdus luminescens genome encoding number of hypothetical proteins, among them probability of coding enzyme function has been reported by conserved domain analysis by sequence and structural homology search. Materials and Methods: All hypothetical proteins were retrieved from the KEGG database with organism code PLU and further checked for their homology with enzyme conserved domain/s using CDDBLAST, INTERPROSCAN, PFAM and CATH program. High scored enzyme coding proteins further analyzed for the homology modeling by PS2 protein structure prediction server. Observation and Results: P. luminescens genome encoding total 353 hypothetical proteins and when they have been searched for enzyme functions, 144 hypothetical proteins engaged with enzyme domains however, only 17 of them posses more than 75% confidence level and further only 15 proteins were able to find the structural homolog. Conclusion: 15 hypothetical proteins of P. luminescens harbors enzyme conserved domains and even show defined sequence and structural homology with known enzymatic protein family and hence reported 15 hypothetical proteins of P. luminescens needs further investigation in wet lab analysis.