ISOLATION OF OUTER MEMBRANE PROTEIN OF AEROMONAS HYDROPHILA RECOVERED FROM CHILDREN WITH DIARRHEA
Journal: BEST : International Journal of Humanities , Arts, Medicine and Sciences ( BEST : IJHAMS ) (Vol.1, No. 2)Publication Date: 2013-11-30
Authors : NASEEM Q N. DUBAI; AZHAR A. L AL-THAHAB;
Page : 15-22
Keywords : Identification A. hydrophila; Isolation of OMP; SDS-PAGE Analysis;
Abstract
A total of 294 stool samples were collected from patient children, their ages were between 1 month to 6 years and suffering from diarrhea disease during period December 2012 and February 2013from Al-Muthana public health laboratory in Muthana province - Iraq. All suspected isolates were screening by traditionally tests and then confirmed by Vitek 2 system and PCR technique(16S r RNA gene).The results showed that; there were 12 (4.08%) positive isolates of Aeromonas hydrophila. The SDS-PAGE method was used to analysis outer membrane proteins (OMPs) profile. The result revealed that, the OMPs molecular weight was around 44 KDa.
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