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ISOLATION OF OUTER MEMBRANE PROTEIN OF AEROMONAS HYDROPHILA RECOVERED FROM CHILDREN WITH DIARRHEA

Journal: BEST : International Journal of Humanities , Arts, Medicine and Sciences ( BEST : IJHAMS ) (Vol.1, No. 2)

Publication Date:

Authors : ; ;

Page : 15-22

Keywords : Identification A. hydrophila; Isolation of OMP; SDS-PAGE Analysis;

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Abstract

A total of 294 stool samples were collected from patient children, their ages were between 1 month to 6 years and suffering from diarrhea disease during period December 2012 and February 2013from Al-Muthana public health laboratory in Muthana province - Iraq. All suspected isolates were screening by traditionally tests and then confirmed by Vitek 2 system and PCR technique(16S r RNA gene).The results showed that; there were 12 (4.08%) positive isolates of Aeromonas hydrophila. The SDS-PAGE method was used to analysis outer membrane proteins (OMPs) profile. The result revealed that, the OMPs molecular weight was around 44 KDa.

Last modified: 2014-01-25 15:55:05