Characterization Of Partially Purified Indigenous Lipase From Germinated Bambara Groundnut Voan Dzeia Subterrenea.

Lipase is an enzyme that catalyzes the hydrolysis of ester bonds in substrates such as phospholipids triglycerides and cholesteryl esters. Lipase E.C. 3.1.1.3 was extracted from the 5 days germinated bambara groundnut Voandzeia subterrenea using 0.1M phosphate buffer pH 7.8. The enzyme was purified using 60 ammonium sulphate saturation via precipitation followed by dialysis and column chromatography using carboxymethylcellulose. The partially purified lipase showed optimum activity at pH 10.0 and temperature of 600C. The Km and Vmax were observed to be 6mm and 1120 UL respectively. Lipase from germinated voandzeia subterrenea has various industrial applications following its stability.