In Vitro Resistant - Induction Of Tamarillo Solanum Betaceum Cav. Applied By UV-B Radiation Against Anthracnose Disease By Determination Of Peroxidase And Polyphenoloxidase Activity
Journal: International Journal of Scientific & Technology Research (Vol.5, No. 6)Publication Date: 2016-06-15
Authors : Elimasni; Dwi Suryanto; Rosmayati; Luthfi A.M.Siregar;
Page : 26-30
Keywords : UV-B Rays; Tamarillo; Solanum betaceum Cav; Peroxidase; Polyphenoloxidase; antrachnose.;
Abstract
The aim of the research was to acquire resistance callus of Tamarillo Solanum betaceum Cav. which was applied by UV-B radiation against the antrachnose disease. The study was designed by Factorial randomized design consisting of two factors UV-B light power 10 20 and 30 watts and exposure time 30 60 and 90 seconds. The Colletorichum filtrate added into medium 25-125 ppm as resistant selection. Callus gained by selection were analyzed by peroxidase and polyphenoloxidase activity changing. Analysis of both enzymes activity showed decreased of protein levels at all samples. But after selection of Colletorichum filtrate both enzymes showed increasing of theirs protein levels. The samples after selection showed more tolerant against filtrate compared to control the highest value obtained in the treatment of UV-B radiation of 20 watts and exposure time of 30 seconds for peroxidase and 20 watt and 60 seconds for polyphenoloxidase. The lowest protein content for both enzyme found on control treatments.
Other Latest Articles
- Toxic And Anti-Cancerogenic Effect Of Brown Seaweed Cystoseira Tamariscifolia
- Optimization Of Scan Range For 3d Point Localization In Statscan Digital Medical Radiology
- Characterization Of Partially Purified Indigenous Lipase From Germinated Bambara Groundnut Voan Dzeia Subterrenea.
- Biochemical Effects Of Aluminum On Some Selected Serum Enzymes Of Male Wistar Albino Rats
- Effectiveness Of UVC Lights Irradiation To Improve Energy Saving
Last modified: 2017-06-11 22:50:08