An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species Dryobalanops Beccarii Dyer

Isolation of high-quality genomic DNA from Dryobalanops beccarii is obviously difficult due to the existence of large amounts of camphor and other secondary metabolites. These contaminants will co-precipitate with DNA during DNA isolation and purification processes and therefore resulting in a brownish DNA pellet that is unsuitable for downstream applications. Many DNA isolation protocols are available for various plant tissues however these protocols are inefficient in yielding high-quality amplifiable genomic DNA especially from camphor containing timber tree species. A CTAB based protocol has been optimized for isolating genomic DNA from camphor containing timber tree species. Key steps include 1 using 1 26946-mercaptoethanol and 2 PVP 40 Mr 40000 in the extraction buffer 2 sample incubation time 40 minutes at 65C and 3 DNA precipitation at room temperature 25C. The isolated DNA pellet was transparent colour and the purified genomic DNA is suitable for PCR amplification.