Study And Molecular Investigation Of Transmembrane Channel-Like1 Gene Related Polymorphic Markers In Iranian Population

Transmembrane channel-like TMC gene has been already reported to cause nonsyndromic autosomal dominant and recessive hearing loss. Finding appropriate genetic Markers for mutation screening of the gene is crucial. The genetic information and population data for these STRs may be used not only in quantitative fluorescence-polymerase chain reaction assays but also in forensic studies and other genetic tests. In this study the identity and characteristics of three CA short tandem repeat STR markers including D9S1876 D9S1837 and D9S1799 related to this gene region were examined for further analysis in the Iranian population. Methods The loci were genotyped by fluorescent capillary electrophoresis DNA sequencing. Results Pair-wise linkage disequilibrium LD showed a considerable LD in paring markers Of D9S1876-D9S1837 and D9S1837-D9S1799. Based on haplotype analysis eleven Informative haplotypes within markers with more than 5 frequency were observed in Iranian population. Conclusion The introduced markers could be suggested as informative and reliable tools in Running linkage analysis of TMC1 gene mutations in the Iranian population.