Gaultherin Production From Gandapura Gaultheria Fragantissima By Enzymatic Inactivation Of Gaultherase

Gaultherin is the active form of salicylate from plants Gandapura. Gaultherin has some characterictics which make it potential to become a natural aspirin anti-cancer antiinflamatory dan cardiopulmonary. Currently aspirin acetylsalicylic acid is a medicine which is used by most of the people in this world because of its function as antipiretic antiinflamatory and analgesic. Approximately the need of pharmacy industry towards gaultherin will be increased in the following year. However at the time being there is still no any effective methods to produce gaultherin from gandapura. This difficulty in the process of taking gaultherin is based on the process of its extraction where the tissue is broken so gaultherin will be hydrolyzed change to be its individual components like methyl salicylate and disaccharides. The hydrolysis process is believed to be catalyzed by the enzyme gaultherase inside. This research is aimed to analyze the production of gaultherin form gandapura using the gaultherase enzyme inactivation process through extraction with alcoholic solvent and determine the correct condition to get the highest production of gaultherin. The result of the calculation shows that the bioextraction process variables of gaultherase enzyme inactivation which is mostly influential are pH and alcohol concentration. The more pH extraction will increase the outcome of gaultherin active compounds The optimum condition of bioextraction enzyme inactivation is in pH 8 with 1446 gaultherin active compounds and regression equation in . The bigger solvent concentration the more gaultherin be extracted. The production of gaultherin will optimally reached in the 90 concentration of ethanol with the result of 1310 active compounds