EVALUATION OF ANTIBACTERIAL EFFECT OF PRODIGIOSIN PIGMENT PRODUCED BY SERRATIA MARCESCENS AGAINST STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI ISOLATED FROM PROCESSED FOOD
Journal: Indo American Journal of Pharmaceutical Sciences (IAJPS) (Vol.04, No. 05)Publication Date: 2017-05-07
Authors : Parya karbaschi; Bahareh Rahimian Zarif; Zahra Houshmandi;
Page : 1348-1353
Keywords : Prodigiosin; Serratia marcescens; Staphylococcus aureus; Escherichia coli; processed food; antimicrobial;
Abstract
Prodigiosin pigment is three Pyrrole that is secondary metabolite produced by some bacterial species like Serratia marcescens . prodigiosin activities and frequent drug shown to inhibit bacterial And has attracted the attention of many researchers in the field of medicine and pharmacy should be.The aim of this study was Evaluation of antibacterial effect of prodigiosin pigment produced by Serratia marcescens against Staphylococcus aureus and Escherichia coli isolated from processed food. Method:Standard strains of Serratia marcescens PTCC 1111 were prepared from collection of microbial strains by Scientific and Industrial Research Organization. Staphylococcus aureus and Escherichia coli strains was isolated and identified by dilution and standard microbiological methods from processed foods. Pigment produced by Serratia marcescens was extracted by the acidic methanol. Finally, by well diffusion, MIC and MBC methods antimicrobial effects of pigments were investigated. Results:Inhibition zone against Staphylococcus aureus and E. coli was 10 mm and 5 mm, respectively. The MIC was 0.78 mg/ml and 3.126 mg/ml for Staphylococcus aureus and E. coli, respectively. MBC was 3.126 mg/ml and 6.25 mg/ml for S. aureus for E. coli, respectively. Conclusion:The S. marcescens prodigiosin produced by showed bactericidal and bacteriostatic effect showing promising antimicrobial activity and suggesting future studies regarding its applicability in antibiotics therapies. In addition, results showed that prodigiosin could be use in foods industry. Key words: Prodigiosin, Serratia marcescens, Staphylococcus aureus, Escherichia coli, processed food, antimicrobial
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