The effect of synthetic estrogen on prooxidant-antioxidant system indexes on different age rats’ organs in vitro

Steroid hormones are synthesized in all living organisms from plants to mammals and that are important for the development and functioning of many organs. This effect mainly arises from the basic regulation of genes that are localized in the nucleus, but are not limited to these genes. Besides, steroid receptors exist outside the nucleus in many organs and cells, with a no clear impact on normal development, health and disease. Besides, steroid receptors exist outside the nucleus in many organs and cells, with a clear impact on normal development, health and disease. Estrogens are female sex hormones, the chemical nature is steroid. Estrogens have multilateral effect on metabolism. Estrogens are spread through the blood to various tissues and because of their lipophilic character can easily diffuse through cell membranes and the blood-brain barrier, exerting effects. In different age periods of life the hormones carry different effects on the body. The most important period of postnatal ontogenesis is puberty, it is called puberty. Nutritional estrogen exposure caused an intensification of lipid peroxidation in animals in pubertal period and mature females. There was a different degree of intensification peroxidation depending on age and investigated organ. The aim of the presented work was to determine the mechanisms of synthetic estrogen influence on the indexes of prooxidant-antioxidant system of different age rats' organs in vitro. Age of experimental animals was 4,.5 months - the pubertal period (group II, n = 6) and 7,5 months - mature ones. Control groups were intact appropriate age animals. In vitro model drug "Sinestrol" which for biological and medicinal properties similar to steroid hormones estrogen were added to test samples in concentration of 0,5 nmol per L, followed by incubation for 1 hour. Research materials were used as serum, brain, liver and kidneys of rats. The data were processed by standard methods of assessing the variation series. In vitro estrogen exposure caused a reduction of lipid peroxidation in the tissues of the studied females in pubertal period and mature rats by activating of antioxidant system by thiol groups increasing. In pubertal females antioxidant system response exceeded the mature animals' response of due to their biochemical processes lability. These data suggest antioxidant defense mechanism of estrogen, which is independent of receptor binding: at the cellular level the effects of estrogen are associated with intracellular signaling pathways and antioxidant enzymes.