Saccharification of Alkaline Treated Rice Straw by Subsequently Hydrolysis of Xylanolytic-Cellulolytic Enzymes for Xylooligosaccharides and Glucose Production

Rice straw (RS) is a biomass found abundantly in Thailand as a waste product from rice grain collections. This study aimed to produce xylooligosaccharides and glucose from RS. The xylan saccharification of aqueous ammonia treated RS was done by a xylanolytic enzyme cocktail (Xyn10E and GH43B6). The analysis of the final liberated product showed that a majority of xylobiose and minority of xylose and xylooligosaccharides were observed. The cellulose saccharification was subsequently conducted by a cellulolytic enzymes cocktail (Cel9R, Cbh9A, CglT). The synergistic action of the cellulolytic enzyme on alkaline treated and xylanolytic hydrolyzed RS liberated sole glucose as a final product. This is an alternative carrier for the total utilization of RS. The subsequent xylanolytic-cellulolytic enzyme hydrolysis of RS to its subunits leads to a potential feed stock for high value products production.