Development of DNA identification for sturgeon species with the use of real time polymerase chain reactionJournal: Fisheries Science of Ukraine (Vol.40, No. 2)
Publication Date: 2017-06-30
Authors : V. Spyrydonov;
Page : 60-67
Keywords : DNA-identification; real time polymerase chain reaction; mitochondrial DNA markers; sturgeon species;
Purpose. Sturgeon is the common name for the 27 species of fish belonging to the family Acipenseridae. The family is grouped into four genera: Acipenser, Huso, Scaphirhynchus and Pseudoscaphirhynchus. Four species may now be extinct. Two closely related species, Polyodon spathula (paddlefish) and Psephurus gladius (Chinese paddlefish, possibly extinct) are of the same order, Acipenseriformes, but belong to the family Polyodontidae and are not considered as "true" sturgeons. The aim of our paper is to develop and carry out a new DNA identification methodology for the sturgeons species with the use of real time polymerase chain reaction. Methodology. A PCR with the detection of results in real time was used for DNA-identification of sturgeon species. Findings. The new real-time PCR was developed and tested for rapid and accurate identification of DNA extracted from eggs and meat of four sturgeon species such as beluga (Huso huso, Linnaeus), Russian sturgeon (Acipenser gueldenstaedtii, Brandt), sterlet (Acipenser ruthenus, Linnaeus) and stellate sturgeon (Acipenser stellatus, Pallas). Based on the results of analytical specificity and comparison of the amplification parameters, we detected specific amplification and absence of cross reactions for fin fragments and eggs of the studied fish species. The proposed DNA-identification method can be used for the identification of sturgeon species that will allow controlling the production of competitive aquaculture products, which will meet CITES requirements. Originality. For the first time, the development and approbation of the methodology for species identification of sturgeon fish by using real-time PCR was carried out. Practical value. Species identification of sturgeon fish will allow controlling the production of competitive and legalized aquaculture products that meet CITES requirements as well as will ensure the implementation of the country strategic plan for food security in Ukraine.
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