THE ALGORITHM FOR DETERMINING THE FUNCTIONAL IMMUNOMORPHOLOGY IN RABBITS SPLEEN
Journal: Paradigm of knowlege (Vol.23, No. 3)Publication Date: 2017-07-11
Authors : Dunaievska O.F.; Vasylchenko V.S.;
Page : 27-36
Keywords : spleen; morphology; immunology; relative area; white pulp; lymphoid nodule; diameter; Kernohan` index;
Abstract
The study focused on determining the functional immunomorphology of rabbit's spleen. The subject of the study was spleen of clinically healthy adult rabbits the california breed at 6-8 months of age, of both sexes. The material was fixed in formalin and poured in paraffin for histological and morphometric studies. The histological preparations are dyeing to hematoxylin and eosin according to Van-Gison`s technology and investigating in a light microscope for study the structure of cells and tissues. A number of quantitative indicators were identified: the relative area of stroma was equal 5,87±0,69 %, the white pulp – 17,68±4,40 %; the ratio of stroma to parenchyma was 33,20 %; the diameter of lymphoid nodule was 240,01±72,32 micrometers; the diameter of light center – 122,43±49,24 micrometers; the germinative-follicular` index was 57,70 %; the width of lymphoid sheaths near the vessels – 39,24± 26,03 micrometers; the lymphoid coefficient – 3,12; the Kernohan` index for the central artery in lymphoid nodule was equal to 1,44±0,34 and 1,26±0,59 for arteries of lymphoid sheaths near the vessels. The results are used for investigating the influence of various factors, immunomodulators and rabbits breeding for produce safeness feed.
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