METAGENOMİCS SEQUENCİNG METHODOLOGİES: REVİEW

Metagenomics can be done by opting different approaches for different samples. Even for the same sample, DNA extraction procedures can be different. Due to these approaches, the concentration and purity of the DNA could be variable. Due to the availability of the different procedures for the metagenomics, the standardization of a specific method cannot be implied directly. There is no specific protocol available which can be followed as a standard protocol for the metagenomics data analysis. Every research problem has been adapted different techniques to solve it. This new branch of science put forth the direct method to gain maximum knowledge of unknown microbial communities. But every time there are chances of getting new diversity as per the sample collection protocol, DNA extraction methodologies, sequencing and data analysis approaches. Metagenomics prove to be a complex science as per the interdisciplinary studies involved. Sanger Sequencing to Big Data Analytics, step by step advancement in the strategies differentiate setup scale and end product analysis for DNA sequencing methodologies. Present data will try to review the available metagenomics methodologies with their applications and drawbacks.