UV SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF CHLORTHALIDONE AND NEBIVOLOL HYDROCHLORIDE IN THEIR COMBINED DOSAGE FORMS

A simple, accurate, precise, reproducible and economical UV spectroscopic method for simultaneous estimation of chlorthalidone and nebivolol hydrochloride in marketed formulation is developed. Method employs solving of simultaneous equations based on the measurement of absorbance at two wavelengths 226 nm and 282 nm which are the λmax values of chlorthalidone and nebivolol hydrochloride in methanol. The linearity of method was investigated in range of 5-25 μg/mL for chlorthalidone and 5-25 μg/mL for nebivolol hydrochloride respectively. The results of analyses have been validated statistically for linearity, accuracy and precision, ruggedness, LOD and LOQ of the proposed method. This method can be successfully employed for simultaneous estimation of all drugs in commercial products.