EVALUATION OF ANTIOXIDANT PROPERTIES CHERRY FRUIT EXTRACT ON PREVENTION THE FAILURE OF DNA

Free radicals preface can result in many diseases such as atherosclerosis, heart diseases, geriatrics and cancer. These diseases are resulting from irregular production of ROS and unstable mechanism of antioxidant protection system.in order to reduction of harmful effects of ROS, natural antioxidants of plants and synthesis antioxidant can be used. It is shown that phenyl compounds, lipid peroxidations and oxidative damage to DNA in some plants can be prevented through cleaning some ROS such as Hydrogen peroxide and hydrocele radicals. Black Cherry is brimful of these compounds; in addition, root and stem of black cherry are used in medical profession. Since genome protection is necessary for demonstration of disease prevention resulting from genome damage, in this study protection in of black cherry as a source of antioxidant in prevention of DNA break is considered. In this study pbr322 plasmid and a nucleotide sequence reproduced from NOS gene were chosen as a sample of PCR product. DNA samples were placed in the vicinity of black cherry juice different density. Conclusion of black cherry juice with density of 10% volume had the most prevention influence on plasmid break. Black cherry juice in both vicinity of and volume in time of 1 hour prevented PCR product break. Our results in this study show that black cherry juice prevents DNA break and destruction of it. Upper vicinity of black cherry juice have been shown that it is possible , because of PH changes , presence of vitamin c and usefulness of peroxide phenyl compounds exist. Key words: DNA damage, Fenton reaction, black cherry juice, nitric oxide.