Genetic diversity of Iranian melon cultigens revealed by AFLP markers
Journal: International Journal of Horticultural Science and Technology (IJHST) (Vol.2, No. 1)Publication Date: 2015-06-01
Authors : Mansoureh Danesh; Mahmoud Lotfi; Shiva Azizinia;
Page : 43-53
Keywords : Amplified fragment length polymorphis; cucumis melo; genetic distance; molecular markers;
Abstract
Iran is a part of the secondary centre of origin of melons, and therefore, there is a wealth of genetic variation of these species in this country. The Amplified Fragment Length Polymorphism (AFLP) marker was applied to investigate the genetic variations among five major Iranian melon cultigens (Cucumis melo) and ‘Ananasi’ as a general well-known cultivar. Ten primer pairs were used on 90 individuals producing 318 polymorphic fragments, with an average of 31.8 fragments per primer combination. The polymorphism rates ranged from 80 to 100%. The genetic similarities among accessions were calculated according to Dice’s Similarity Index and used to construct a dendrogram based on the Unweighted Pair Group Method with Arithmetic Average (UPGMA). The genetic distance estimates based on AFLPs ranged from 0.29 to 0.63, with a mean of 47±0.3. Iranian melon genotypes and the ‘Ananasi’ cultivar were considered as two separate groups on the cluster analysis. The principal coordinate analysis showed a separate allocation of the melon cultivar groups. The results demonstrated a wide diversity of Iranian melon cultigens. The high number of alleles and the high expected genetic diversity detected with the AFLP marker indicated that the Iranian melon cultigens had distinctive characteristics and were an important genetic diversity pool, which made them a valuable source of breeding materials.
Other Latest Articles
- Isolation and Sequence Analysis of GpdII Promoter of the White Button Mushroom (Agaricus bisporus) from Strains Holland737 and IM008
- Orchard Management for Decreasing Date Palm Bunch Fading Disorder
- Effect of postharvest calcium chloride treatment on the storage life and quality of persimmon fruits (Diospyros kaki Thunb.) cv. ‘Karaj’
- Saltgrass, a potential future landscaping plant and a suitable species for desert regions: A review
- Paclobutrazol-Induced Biochemical Changes in Pomegranate (Punica granatum L.) cv. ‘Malas Saveh’ under Freezing Stress
Last modified: 2017-08-15 23:19:48