SIMULTANEOUS ESTIMATION OF EMITRICITABINE, EFAVIRENZ, TENOFOVIR DISPROXIL FUMARATE BY CHEMOMETRY AND REVERSE PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

A chemometric ultra-violet spectrophotometric method of analysis, Partial Least Square (PLS) method was applied to simultaneous assay of emitricitrabine, efavirenz and tenofovir disoproxil fumarate (DF) in their combined dosage tablet formulation. For comparison of this chemometric method a HPLC method for simultaneous determination of emitricitabine, efavirenz and tenofovir DF in combination was developed. Twenty one mixed solutions were prepared for chemometric calibration set and twenty one ternary mixtures were prepared as validation sets. The absorbance data matrix for training set was obtained by recording absorbance within wavelength range 230-290 nm at 2nm intervals. The developed multivariate and HPLC methods were successfully tested for laboratory mixtures as well as commercial tablet formulation of emitricitrabine, efavirenz, tenofovir DF.