DEVELOPMENT OF ULTRAVIOLET SPECTROPHOTOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF BRIMONIDINE TARTRATE AND BRINZOLAMIDE

A simple, accurate and precise UV spectrophotometric method has been developed for the simultaneous estimation of brimonidine tartrate (BRT) and brinzolamide (BRZ) using methanol as solvent. The λmax for BRT and BRZ were found to be 244 nm and 253 nm, respectively. The linearity was found in the range of 1-7 μg/mL and 5- 35 μg/mL for BRT and BRZ, respectively. The LOD and LOQ for BRT were found to be 0.0149 µg/mL and 0.0425 µg/mL at 244 nm and 0.0278 µg/mL and 0.0843 µg/mL at 253 nm, respectively whereas those for BRZ were found to be 0.0330 µg/mL and 0.1000 µg/mL at 244 nm and 0.0243 µg/mL and 0.0737 µg/mL at 253 nm, respectively. The % recovery of the drugs by the proposed method was found in the range of 98.29% to 100.82%. The method was found to be precise and robust. The method was successfully applied for determination of BRT and BRZ in an in-house suspension.