Optimized Synthesis of Gold Nanoparticles using Green Chemical Process and its Invitro Anticancer Activity Against HepG2 and A549 Cell Lines

In the present study, the biosynthesis of gold nanoparticles (AuNPs) was achieved by using marine brown seaweed Padina tetrastromatica (PT) as a reducing and capping agent. The optimized Au Nps were performed by changing the concentration of algae extract, pH and temperature was analyzed by UV-vis spectrophotometer. The synthesized Au NPs were characterized byXRD, FTIR, SEM, TEM, EDX and SAED. The X-ray diffraction showed the Au NPs which can state by the presence of peaks at (1 1 1), (2 0 0), (2 2 0) and (2 2 2). The FTIR result clearly showed that the extracts containing -OH as a functional group (sugar molecules) act in capping the nanoparticles synthesis. SEM images revealed that all particles were spherical in shape. TEM image confirms the spherical in shape with an average size ranges from 8-10 nm. Synthesized Au NPs were evaluate for the in vitro cytotoxic activity of human liver cancer (HepG2) and lung cancer (A549) cell line at the different concentrations compared with standard drug cyclophosphamide.