Phyto-Assisted Synthesis of Silver Nanoparticles using Solanum Nigrum and Antibacterial Activity Against Salmonella Typhi and Staphylococcus Aureus

This study aims to provide a simple and eco-friendly approach for the synthesis of Ag NPs using medicinal plant Solanum nigrum aqueous leaf extract. Fresh leaf extract mediated the reduction of silver from higher excited state to the ground extract when mixed with 1 mM silver nitrate solution. Reduction led the synthesis of silver nanoparticle with the size range of 20-40 nm was confirmed by TEM analysis. UV-Vis analysis demonstrates the synthesis of Ag NPs by its standard peak due to SPR (Surface Plasmon Resonance). XRD analysis confirmed the crystalline nature of Ag NPs. It was further characterized by FT-IR for the confirmation of functional groups responsible for Ag NPs synthesis and elements analyzed using EDX. Our study also showed the high antibacterial effect of nanoparticle against disease causing virulent bacterial strains (Salmonella typhi and Staphylococcus aureus).