ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF NEW UFLC METHOD FOR DETERMINATION OF QUERCETIN IN ALCOHOLIC AND AQUEOUS EXTRACTS OF CAMELLIA SINENSIS

A precise, simple, responsive and feasible ultra-fast liquid chromatography (UFLC) method for novel analysis of quercetin in aqueous and alcoholic extracts of Camellia sinensis with internal standard has been developed. The analysis was carried out on a Phenomenax-C18 column (250 × 4.6mm, 5μm). Here, acetonitrile and orthophosporic acid of 0.1% V/V in water at a 60:40 ratio was used as the mobile phase and propyl paraben was used as the internal standard. The linearity range of quercetin was found to be between 1-25 µg/mL and retention times of quercetin and propyl paraben were found to be 3.3 and 5.6 min, respectively. In this developed method, accuracy and recovery values of quercetin were found to be good, ranging from 100 to 102% respectively. The proposed current novel analytical method was validated with respect to system suitability, linearity, limit of detection (LOD), precision and limit of quantification (LOQ), accuracy (recovery), specificity and sensitivity in respect to the ICH guidelines. This proposed method can be readily used for determination of quercetin in research institutes and quality control laboratories.