Effective Biosurfactants Production by Pseudomonas aeruginosa and its Efficacy on Different Oils
Journal: Journal of Advanced Laboratory Research In Biology (Vol.1, No. 1)Publication Date: 2010-07-01
Authors : K. V. Sekar; Sarita Kumari; A. Nagasathya; S. Palanivel; Subramanyam Nambaru;
Page : 31-34
Keywords : Biosurfactant production; Pseudomonas aeruginosa; Rhamnolipid; Test efficacy;
Abstract
A rhamnolipid producing bacterium, Pseudomonas aeruginosa was isolated from contaminated soil with oily wastes. The Pseudomonas aeruginosa grown with glucose and corn oil as a carbon source produced bio-surfactant. This bio-surfactant was purified by procedures that included chloroform-ethanol extraction and 0.05M bicarbonate treatments. The active compound was identified as rhamnolipid by using thin layer chromatography. The emulsification activity of bio-surfactant, the coconut oil responded better than the olive oil, groundnut oil and sunflower oil and gave a maximum level of 1cm
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