DYNAMIC STUDY OF PHYSIOLOGICALLY ACTIVE SUBSTANCES SYNTHESIZED BY MICROMYCETES INCLUDED IN BIOPREPARATION «MICROUSTIRGICH»
Journal: International Scientific Journal "Internauka" (Vol.1, No. 34)Publication Date: 2017-08-31
Authors : Turaeva Bakhora; Zukhritdinova Nigora; Azimova Nodira; Karimov Khusniddin; Tokhtakhunova Aziza; Khamidovа Khursheda;
Page : 11-14
Keywords : molasses; micromycetes; indole acetic acid; gibberellin acid;
Abstract
Molasses, which is considered to be industrial waste, was used as carbon and energy source in Chapeka and Mandels nutrient media. The most active synthesis of indole acetic acid (IAA) by T. harzianum Uz CF-55 was 2,4 mg/ml on the 1, 4, 5th days, by P. canescens Uz CF-54 was 1,54 mg/ml on the 1st, 2nd days, by F. moniliforme Uz GC-12 was 1,5-2,4mg/ml on the 1, 2, 3, 4th days of cultivation. The maximum activity of gibberellin acid (GA) synthesized by the fungus T. harzianum Uz CF-55 on nutrient medium containing 5% sucrose and molasses was 3 mg/ml, 0,67 mg/ml and 0,68 mg/ml on the 3rd, 6th and 9th days of cultivation, respectively. F. moniliforme Uz GC-12 fungus cultivated on nutrient medium containing 5% sucrose and molasses was found to be effective on the 3rd day with 0,58 mg/ml, and on the 5th and 6th days with 0,62 mg/ml of GA. P. canescens Uz CF-54 cultivated on the same nutrient medium produced the maximum amount of GA which was 0,69 mg/ml and 0,78 mg/ml on the 9th and 10th days, respectively. Enrichment of the nutritional content of these micromycetic strains by cheap raw, such as molasses, allows to increase the amount of biologically active substances stimulating the growth of the plant and to reduce the net cost of the biopreparation.
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