Approach to analyse kinetics of erythrocyte hemolysis

The hemolytic stability of erythrocytes is studied under various pathological conditions, including ischemia and complications after myocardial infarction. A large set of parameters are used to describe hemolysis, among which the duration of stages, the rate of stage, the percentage of hemolysed cells and others. Diagnostic interpretation of such results is difficult and is of little purpose for clinicians. The characteristics of hemolysis often are the kinetic parameters. A method for analyzing the kinetics of hemolysis is proposed, which makes it possible to obtain additional information on the distribution of erythrocytes by their hemolytic resistance. The method is easily implemented and allows obtaining statistically significant results suitable for automated diagnosis. The developed methodology was applied to the analysis of acid hemolysis of healthy volunteers and patients with cardiovascular pathology. The described approach to the analysis of the process of hemolysis of erythrocytes made it possible to reveal changes in the resistance of erythrocytes to acid-induced hemolysis in people with coronary heart disease and to detect a change in the ratio of the number of erythrocytes with different life span in the circulation.