SIMULTANEOUS EQUATION METHOD FOR THE ESTIMATION OF PALBOCICLIB AND LETROZOLE BY UV-VISIBLE SPECTROPHOTOMETRY

A simple, rapid, accurate, economic and precise UV/VIS method has been developed and validated. Choice of a common solvent were essential so various solvent ranges including methanol, ethanol, acetonitrile and phosphate buffer and various concentrations ranges of various buffers were analyzed. methanol was selected as a solvent for the proposed method. Palbociclib and letrozole showed maximum absorbance at 220 and 240 nm respectively. Both drugs obey Beer Lambert's law in the concentration range of 2-10 μg mL-1 for palbociclib and letrozole respectively. The LOD and LOQ were found to be 0.0782 μg mL-1and 0.2142μg mL-1for palbociclib, respectively. For Letrozole, the LOD and LOQ values were found to be 0.0585 μg mL-1and 1.6510μg mL-1 respectively. The method was quantitatively evaluated in terms of linearity, precision, precision, LOD, LOQ and recovery. The method is simple, convenient and suitable for the analysis of palbociclib and letrozole in bulk drug.