A STUDY ON KINETICS OF BIO-SURFACTANT PRODUCED BY BACTERIAL STRAINS ISOLATED FROM MUNICIPAL WASTEWATER

Bio-surfactants or surface molecules are used to reduce surface tension for both aqueous solutions and hydrocarbon mixtures. In this study, kinetics of bio isolated from municipal wastewater was assessed. The kinetic studies were carried out in batch culture for bio experiments, Critical Micelle Dilution (CMD emulsification, biomass surface tension, were recorded for a period of 84 h. Maximum biomass production (about 3.2g/l dry weight) was found after 60 h of growth. The surface tension of culture dropped rapidly after inoculation and achieving its value (36 mN/ m) during the exponential phase after about 36 ability of strains was evaluated through bio hemolysis test and measurement of surface tension. We also assessed the effect different pH, salinity concentrations, and temperatures for bio Among all important features of the isolated strains, one of the strains (P4: Bacillussp.) showed high salt tolerance and their successful production of surfactant in a vast pH and temperature domain and reduced surface tension to value below 40 mN/m. Microbial enhanced oil recovery can be done through this potential strain. The bio-surfactant component produced by strain P4 was mainly glycolipid in nature.